The Hertie Institute for Clinical Brain Research (Research Division Translational Genomics of Neurodegenerative Diseases, Dept. of Neurodegenerative Disease) is inviting applications for a
PhD student, TVL E13, 65%, 3 years (extension possible)
in multi-omic pharmacodynamic biomarkers for autosomal-recessive ataxias
The Hertie Institute for Clinical Brain Research (HIH), together with the Department of Neurology, forms the Center for Neurology at the University of Tübingen. It is dedicated to basic and translational research in neurological diseases. Together with the several other highly advanced neuroscience institutes, it is part of the TübingenNeuroCampus (TNC), here working closely together also with the German Center for Neurodegenerative Diseases (DZNE). Scientists in the more than 100 active research groups of the TNC pursue theoretical, system-neuroscientific, molecular, and clinical research approaches in their entire breadth using a wide range of methods.
The research division „Translational Genomics of Neurodegenerative Diseases“ of Prof. Synofzik focuses on genomics, pathophysiology and translational biomarker research in the field of neurodegenerative diseases, with a special focus on genetic ataxias, motor neuron diseases, and dementias. Prof. Synofzik coordinates several large trans-European consortia on translational neurodegeneration in rare movement disorders and serves as PI of several large-scale national and international longitudinal cohort studies. We offer excellent projects and training in neurodegeneration and molecular biology research. A wide range of molecular, protein biochemical and cell biological methods are applied in the lab (e.g. exome/genome sequencing, Sanger sequencing, qPCR, Western Blotting, ELISAs, and cutting-edge ultra-sensitive protein analysis including SIMOA and Luminex technology). Biomarkers are identified in cell culture models, human and murine blood and/or CSF, and validated in patient cohorts.
The PhD project: You will identify and validate pharmacodynamic biomarkers in two autosomal-recessive ataxia disorders (ARSACS and COQ8A) using an integrated mouse/human cross-species cross-model pipeline. Fluids (CSF, blood) from mice models and tissue from cell models of ARSACS and COQ8A treated with novel disease-modifying drugs (the corresponding cell and animal work will be performed by collaboration partners and will not be part of this project) will be analyzed with unbiased transcriptomic and proteomic techniques to establish molecular treatment signatures and identify treatment response markers. Biomarkers candidates will be validated in human fluids (CSF, blood) using targeted assays (qRT-PCR, MSD ELISA, and ultrasensitive Luminex and Simoa assays) to ensure direct relevance of findings for the human condition.
Your prior methodological experiences: For this project, you have already worked previously with a broad range of molecular techniques, including: qRT-PCR, Western Blot, ELISA, and ultrasensitive immunoassays. Ideally, you have also already worked with unbiased omics methods (transcriptomics, liquid chromatography–mass spectrometry), but this is not a necessary prerequisite.
This position will be funded by the ‘Bundesministerium für Bildung und Forschung’ (BMBF) via funding for the European Joint Programme Rare Diseases 2020 (EJPRD).
What we are looking for:
- You are creative, highly motivated and you are able to work independently
- You have worked previously with all of the following techniques: qRT-PCR, Western Blot, ELISA, and ultrasensitive immunoassays.
- Ideally, you also already have experience in protein biomarker work - either proteomics or targeted protein assays, in particular on ultra-sensitive protein platform
- Essential qualities include fluency in English and a strong team spirit.
- You have a Master’s degree in Biochemistry, Biology, Bioinformatics, Molecular Genetics, or related life sciences.
What we are offering: We offer a challenging interdisciplinary translational project that is integrated into major national and European research consortia at the interface of genomics and translational medicine, well-equipped laboratories with top-notch facilities, excellent supervision in a highly collaborative international environment and affiliation with the Graduate Training Center of Neuroscience. The position is available immediately. Salary will be determined according to the German collective wage agreement in public service (TVL 13, 65%). Appointment is full time and will be initially for three years with the possibility of renewal. We give priority to severely disabled applicants with essentially equal qualifications.
Application: If you are interested in this project please send your full application within one PDF file. This should include:
- Cover letter outlining (i) how you meet the requirements for the position, (ii) which techniques/methods you would like to be involved in, (iii) relevant details of your past research projects, and (iv) an explanation of how your previous experience lends itself to this PhD research project. (~750-1000 words).
- Curriculum vitae
- Names and email addresses of two professional references (e.g., current or previous research advisors).
- transcripts, your master’s thesis and/or publications.
Please send this PDF to: Mrs Selina Reich (Research Division Prof. Synofzik): selina.reichuni-tuebingen.de
Application Deadline: 20.10.2021
Head, Research Division Translational Genomics of Neurodegenerative Diseases
Department of Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research